Loading...
 

Proteomics Karolinska (PK/KI)

PK/KI is the proteomics core facility at KI located at the department of Department of Medical Biochemistry and Biophysics. We provide services using best available proteomics techniques and methods with focus on label-free quantitative proteomics.

Image

Our main applications:
  • Large scale quantitative proteomics experiment.
  • Accurate molecular mass determination of e.g. peptides and small proteins.
  • Detection of posttranslational modification and their localization on the amino acid sequence.
  • General protein identification and quantitation from different sources.

Contacts

If you are interested in using our services or you wish to discuss your project please contact us:
Akos Vegvari (akos.vegvari@ki.se) (Facility Manager)
Alexey Chernobrovkin (alexey.chernobrovkin@ki.se) (Bioinformatitian)
Carina Palmberg (carina.palmberg@ki.se) (Technical expert)
Marie Ståhlberg (marie.stahlberg@ki.se) (Technical expert)

PK/KI @ SciLifeLab Stockholm:

http://www.scilifelab.se/facilities/amsp/(external link)

Recent Projects:

  • Functional Identification of Target by Expression Proteomics (FITExP) reveals protein targets and highlights mechanisms of action of small molecule drugs. Chernobrovkin A. et al., Sci. Rep. 2015; 5:11176. doi:10.1038/srep11176(external link).
  • Identification of a large protein network involved in epigenetic transmission in replicating DNA of embryonic stem cells. Aranda S et al., Nucleic Acids Res. 2014;42(11):6972-86. Epub 2014 May 22.
  • Binding of Pro-Gly-Pro at the active site of leukotriene A4 hydrolase/aminopeptidase and development of an epoxide hydrolase selective inhibitor. Stsiapanava A et al. ,Proc Natl Acad Sci U S A. 2014 Mar 3.
  • Prognostic Polypeptide Blood Plasma Biomarkers of Alzheimer's Disease Progression. Yang H et al. J Alzheimers Dis. 2014 Feb 6
  • Protein biomarkers in vernix with potential to predict the development of atopic eczema in early childhood. Holm T, et al. Allergy. 2014 Jan
  • Blood plasma IgG Fc glycans are significantly altered in Alzheimer's disease and progressive mild cognitive impairment. Lundström SL et al. J Alzheimers Dis. 2014
  • Urinary prognostic biomarkers and classification of IgA nephropathy by high resolution mass spectrometry coupled with liquid chromatography. Kalantari S, et al. PLoS One. 2013 Dec
  • Wobble decoding by the Escherichia coli selenocysteine insertion machinery. Xu et al., Nucleic Acids Res. 2013 Aug 27. PMID: 23982514.
  • In silico instrumental response correction improves precision of label-free proteomics and accuracy of proteomics-based predictive models. Lyutvinskiy et al., Mol Cell Proteomics. 2013 Aug;12(8):2324-31. doi: 10.1074/mcp.O112.023804. PMID: 23589346.